The application of different sample preparation techniques to improve LC-MS based assay performance

Thursday, 08th September, 2022, 03:00 PM - 04:00 PM CEST.

This presentation will look at a range of sample preparation techniques, including protein precipitation, supported liquid extraction (SLE) and solid phase extraction (SPE), which will address the issues mentioned previously. The presentation will provide an insight to understand how these different techniques work, and how they can be successfully applied to analysing biological fluids. Workflows will be presented that detail how to choose between the different approaches, and with SPE providing an understanding of which stationary phase to select, and how to optimise the methodology. Examples will be provided that demonstrate that a generic approach to method development can be successfully applied, based on an understanding of the physiochemical properties of the analytes.